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algorithms imaris v8 4 1 bitplane n a maxquant v1 5 2 8  (Oxford Instruments)
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algorithms maxquant 1 6 3 4  (Addgene inc)
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scr_002798  (GraphPad Software Inc)
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algorithms maxquant  (Addgene inc)
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Algorithms Maxquant, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peaks studio  (Bioinformatics Solutions Inc)
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proteome discoverer software  (Thermo Fisher)
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Thermo Fisher proteome discoverer software
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algorithms maxquant software mpi biochemistry martinsried  (Addgene inc)
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algorithms image lab software bio rad rrid scr 003070 prism v7 graphpad rrid scr 002798 maxquant platform tyanova  (Bio-Rad)
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Bio-Rad algorithms image lab software bio rad rrid scr 003070 prism v7 graphpad rrid scr 002798 maxquant platform tyanova
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scaffold software version 4.3.2  (Proteome Software Inc)
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Proteome Software Inc scaffold software version 4.3.2
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KEY RESOURCES TABLE

Journal: Cell reports

Article Title: A Systematic Protein Turnover Map for Decoding Protein Degradation

doi: 10.1016/j.celrep.2020.108378

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Abcam Cat# ab290; RRID: AB_303395 Rabbit polyclonal anti-Rpn1 Dan Finley N/A Rabbit polyclonal anti-Rpn5 Dan Finley ( Shi et al., 2016 ) N/A Rabbit polyclonal anti-Rpn8 Dan Finley N/A Rabbit polyclonal anti-Rpn12 Dan Finley N/A Mouse anti-IgG kappa binding protein-HRP Santa Cruz Biotechnology Cat# sc-516102; RRID: AB_2687626 Mouse monoclonal anti-rabbit IgG-HRP Santa Cruz Biotechnology Cat# sc-2357; RRID: AB_628497 Mouse monoclonal anti-Pgk1 Invitrogen Cat# 459250; RRID: AB_2532235 Mouse monoclonal anti-FLAG Sigma-Aldrich Cat# F1804; RRID AB_262044 Chemicals, Peptides, and Recombinant Proteins heavy [ 13 C 6 / 15 N 2 ] L-lysine Cambridge Isotope laboratories, Inc. CNLM-291-H-PK Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base US Biological Cat# D9515B Critical Commercial Assays SuperSignal West Pico SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 SuperSignal West Dura SuperSignal West Dura Thermo Fisher Scientific Cat# 34076 SuperSignal West Dura Thermo Fisher Scientific Cat# 34076 Deposited Data Mass spectrometry proteomics data Proteomics Identifications Database (PRIDE) repository PRIDE: PXD008706 Experimental Models: Yeast strains ( Saccharomyces cerevisiae ) BY4743 Dharmacon N/A BY4741 Dharmacon N/A BY4741; pep4Δ::kanMX Dharmacon N/A BY4741; CHS3-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX CHS3-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; FCY2-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX FCY2-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; CPR4-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX CPR4-TEV-6xGly-3xFLAG::hphMX This study N/A Recombinant DNA Plasmid: pTF272 (TEV-6xGly-3xFLAG) Tim Formosa Addgene #44083 Software and Algorithms MaxQuant (version 1.5.2.8) ( Tyanova et al., 2016 ) https://www.maxquant.org/ Perseus (version 1.5.1.7) ( Tyanova and Cox, 2018 ) https://maxquant.net/perseus/ TreeView ( Saldanha, 2004 ) N/A Open in a separate window KEY RESOURCES TABLE RESOURCE AVAILABILITY Lead Contact Further information and requests for resources and reagents generated in this study should be directed to and will be fulfilled by the Lead contact, Tobias C. Walther ( ude.dravrah.hpsh@rehtlawt ).

Techniques: Binding Assay, Recombinant, Mass Spectrometry, Plasmid Preparation, Software